CYTOTOXIC ACTIVITIES OF GLYCOALKALOID SOLAMARGINE AGAINST GASTRIC CANCER CELL

Alana Sertore Villela Caldas, Raquel Alves dos Santos, Rommel Rodrigues Burbano, Jairo Kenupp Bastos, Cristiane Fernandes de Freitas Tavares

Resumo


Purpose: The gastric carcinoma is among the main neoplasms related to incidence and mortality, especially in the state of Pará, Brazil, with high prevalence of this pathology. The Solamargine is a glycoalkaloid has been isolated from the fruits of Solanum incanum indicated significant cytotoxic activities with activation of cell death (apoptosis) the carcinoma cells. The objective of this present study will be to evaluate the cytotoxicity of Solamargine in gastric cancer cell line (AGP-01). Methods: The Solamargine was purified from Solanum incanum acoording to the procedures previous (Lin et al, 1990). The cell AGP-01 line were maintained in DMEM +HAM F10 medium supplemented with 10% fetal bovine serum, 2 mM glutamine, 100 U/mL penicillin, and 100 mg/mL streptomycin at 37 °C with 5% CO. The AGP-01 cells will be treated with a constant concentration of solamargine (2, 5 and 10 mg/mL) at 37° for 24 hr. The cytotoxicity of solamargine against AGP-01 cells will be determined by XXT method. The IC50 will be calculated from the Solamargine concentration that induced a 50% of the cell death. Results: At the moment the tests are being performed and analyzed. Conclusion: Thus, the researches suggest that Solamargine has potencial antiproliferative activity against the tumor cell lines, which may contribute to the description of new drugs for the treatment the gastric carcinoma.


Palavras-chave


apoptosis, gastric cancer, solamargine.



DOI: http://dx.doi.org/10.26843/investigacao.v16i7.2283